Residential course for technicians and graduates in the medical, veterinary and biological sector, with specific experience and in-depth knowledge in the genetics of animal cells. The course provides in-depth analysis and discussions on the main themes and advanced methodologies in the generation of transgenic mice and in the freezing of embryos.
Scientific Organisers
Fiorella Altruda, Emilio Hirsch, Valeria Poli
Research Center for In Vivo Functional Genomics
c/o Molecular Biotechnology Center (MBC), University of Turin
Lecturers
Fiorella Altruda, Ornella Azzolino, Flavio Cristofani, Sharmila Fagonee, Emilio Hirsch, Emilia Turco, Maddalena Iannicella, Erika Messana, Noemi Morello, Valeria Poli, Antonella Sgarra, Emanuela Tolosano, Emilia Turco
Research Center for In Vivo Functional Genomics
c/o Molecular Biotechnology Center (MBC), University of Turin
COURSE PROGRAMME
Monday 12th October 2009
9.00 - 9.15 Registration and welcome of participants
9.15 – 13.00 Experimental strategies for the preparation of DNA constructs
- 09.15 - 10.15 Transgenic Mice, F. Altruda
- 10.15 - 11.15 Gene Targeting, E. Hirsch
- 11.15 - 11.30 coffee break
- 11.30 - 12.30 Conditional Mutants, V. Poli
12.30 - 14.00 Lunch
14.00 - 14.45 Targeting vector preparation by homologous recombination in bactery, E. Turco
14.45 - 18.00 Beginning to culture ES cells
teachers: O. Azzolino
- Preparation of the feeder layer
- Electroporation
- Starting the culture of ES cells
Tuesday 13th October 2009
9.00 - 13.00 Selection of Colonies
teachers: O. Azzolino – E. Messana
- Oocyte sampling
- Observation of the ES cell cultures
- ES cell colony picking
13.00 – 14.00 Lunch
14.00 - 18.00 Gene Transfer
teachers: M. Iannicella – A. Sgarra
- Microinjection into oocytes
- Reimplantation of oocytes
- Preparation of vasectomized mice
Wednesday 14th October 2009
9.00 - 12.00 Microinjection Techniques
teachers: O. Azzolino – A. Sgarra
- Blastocyst Sampling
- Preparation of capillaries for microinjection
12.00 - 13.00 The phenotype of mutants studied by the stereomicroscope
13.00 - 14.00 Lunch
14.00 - 18.00 Reimplantation
teachers: O. Azzolino – E. Hirsch – A. Sgarra
- Microinjection of ES cells into blastocysts
- Reimplantation of blastocysts
- Extraction of ES cell DNA
Thursday 15th October 2009
9.00 - 13.00 Analysis of ES cell DNA
teachers: E. Messana – N. Morello
- Freezing of ES cells
- Continuation of ES cell extraction
- Freezing of embryos
13.00 - 13.30 CME test and conclusion
GENERAL INFORMATION
The course will be held in the laboratories of the Research Center for In Vivo Functional Genomics (MBC) at the University of Turin in Via Nizza 52.
The registration form, must be sent to elisabetta.lombardini@fobiotech.org together with a CV by 20th of September 09.
The course is organised for a limited number of participants. If the number of applicants exceeds the number of available places, a selection of participants will be based on CVs.
The registration fee is € 720,00 (€ 600,00 + V.A.T. 20%), including coffee breaks, lunches and study material. This fee must be paid by a bank transfer or by credit card after having received confirmation of acceptance from the organising secretary. Payment instruction available on www.fobiotech.org
Bank Transfer Details
Header Fondazione per le Biotecnologie
Bank Intesa San Paolo
Branch Agenzia 01
Branch Address C.so Re Umberto I, 51/53 10128 Torino, Italy
Account number 100000104365
IBAN IT81 Q030 6901 0011 0000 0104 365
SWIFT code IBSPITTM501
BIC BCITITMM
The title of the chosen course and the surname of the participant must be specified in the payment.
If, after registration and payment, you cannot participate, please be aware that you must inform the organizing secretariat at least 15 days before the beginning of the course in order to have 70% of the registration fee reimbursed.
No reimbursement will be given for communications after this deadline.
CME credits for Italian and European participants will be soon requested by the Italian Ministry of Health and by the European Accreditation Council for the following professional categories: general practitioner (medico), biologist (biologo), chemist (chimico), veterinarian (veterinario) and laboratory technician (tecnico sanitario di laboratorio biomedico).
Please note that CME (Continuous Medicine Education) credits will be given only to Health Care professionals/personnel working in the Health Care sector (both private and public) as employees or self employed persons.
For further information please contact
Elisabetta Lombardini
Fondazione per le Biotecnologie
e-mail: elisabetta.lombardini@fobiotech.org
Tel. 011 6600187 – fax. 011 6600708
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